I have been doing a lot of Western blots lately and thought that I would show a picture of one so that you at least have an idea of what I am talking about. The blot I am showing you is actually three blots - one looking at phopho-AKT, another at phospho-JNK, and the last (far right) at phospho-ERK. There are two lanes in each blot, one is of tumor sample, and the other of control tissue. This has been my life the last couple of weeks. The tumor samples are showing darker bands but that is probably because there is more protein in the tumor lanes than in the control lanes rather than an actual upregulation of enzyme activities.
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