ChIP update

Science moves at a snail's pace most times. I finally have the first part of this experiment nailed down (DNA isolation and cleavage). I was using a sonicator to break up the DNA but was getting poor results. I went to an enzymatic method called Micrococcal Nuclease digestion and have had some success. I am going to run through this once more tomorrow and attempt to clean the results up by combining the MNase digestion with sonication. Next week I will move on to infecting the cells with virus so that p75 will be expressed and then work on getting p75 binding to beads for the immunoprecipitation part of the experiment. Once I have that worked out, I will change cell types and make whatever small changes I need to make before really hitting this experiment for results.